3rd Microfluidics Webinar October 23-23, 2021 London, UK

Biography:

Dr. Muhammad Asif has completed his PhD at the age of 31 years from Huazhong University of Science and Technology (HUST), China and postdoctoral studies from School of Chemistry and Chemical Engineering, HUST. Currently, he is postdoc fellow in School of Materials Science and Engineering, Wuhan Institute of Technology, China. He has published more than 50 papers with commulative Impact Factor of 346, in reputed journals like BB, AC, CEJ, ACS-AMI, SNB, advances in colloid and interface science and has been serving as an editorial board member of Frontiers in Chemsitry, Frontiers in Analytical Science.  

Abstract:

The development of molecular-scale hybridized template of vertically stacked 2D superlattice materials with tunable interior architectures holds crucial role in various promising technologies, but the molecular-scale alternate stacking in hybrid material could be much more challenging. Herein, we have established a self-assembly of periodic superlattice material by integrating positively charged semiconductive sheets of Zn-NiAl layered double hydroxide (LDH) with reduced graphene oxide (rGO) layers known to be negatively charged by controllable co-feeding protocol and explored its practical applications in ultrasensitive, discriminative, and simultaneous detection of early diseases diagnosis biomarkers including dopamine (DA), uric acid (UA) and ascorbic acid (AA). Due to the harvested synergistic effect of drastic interfacial conduction imparted by direct neighboring of conductive graphene to semiconductive channels of LDHs in heteroassembly, superb intercalation feature of LDHs and enlarged surface area with enormous surface active sites, Zn-NiAl LDH/rGO modified electrode presents incredible electrocatalytic activity towards the oxidation of these biomolecules. The proposed biosensor has revealed outstanding electrochemical performances in terms of good selectivity, ultrasensitivity, broad linear ranges and low real detection limit of 0.1 nM and 0.9 nM for DA and UA, respectively. And its successful utilization in electrochemical sensing system for real-time tracking of neurotransmitter DA efflux from live human nerve cells has been validated. Therefore, our molecular-scale hybridized superlattice strategy using graphene as π-electron-rich substrate to modulate electronic structure of LDHs will open new horizon in material engineering, biosensing platform and pathological diagnostics.

 

Biography:

Kenia Chávez graduated with honors from the BSc. Chemistry in 2014 at UNAM. In 2017, she has completed her master’s degree at the School of Chemistry (UNAM) and began her PhD studies. Her current research includes a multidisciplinary project with the intention of developing microdevices focused on clinical diagnosis through the detection and quantification of antibodies present in blood plasma by means of an Enzyme-Linked Immunosorbent Assay (ELISA).

 

Abstract:

In recent decades, scientific interest in the development of devices capable of performing routine clinical analyzes through the application of standardized traditional laboratory protocols in a miniaturized lab-on-a-chip device has increased. In the present work, an innovative microdevice for the on-line whole blood dilution with a phosphate buffer solution (PBS) and separation of plasma was designed, manufactured, and characterized. The microdevice was constructed with a rectangular cross-section and spiral-shaped microchannels by photolitography and soft litography. Also, the widths of the diluted plasma and the remaining blood outlet microchannels were different to create a difference in the outlet flowrates to facilitate and achieve the plasma separation based on the combination of centrifugal effect (Dean drag force) and bifurcation law (Zweifach-Fung effect). The separation purity (a) under the separation conditions (total flowrates between 25 and 100 µL/min, entrance flowrate ratio PBS/whole blood between 4 and 10, and hematocrit (% HCT) between 3 and 8) were around 100 % for fresh blood samples, while the separation efficiency (β) was between 8 and 13 %. The concentration in the separated diluted plasma was between 0.1 and 0.7 % (v/v) with plasma flowrates between 3 and 7 µL/min, respectively. The quality of the diluted and separated plasma from micordevice was corroborated from a blood sample from a patient diagnosed with rheumatoid arthritis through the quantification of anti-cyclic citrullinated peptides (anti-CCP) antibodies employing a microdevice immunoassay. The developed microdevice has a high potential to be coupled with the on-line detection of biomarkers.